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Aaa B 7 greement n" 228330 An approach towards a methodology which produces device-independent images and allows better reproducibility and inter-comparisons between images, centres on three aspects: 1. Understanding the experimental factors (such as the limitations of commercially available equipment) and the physical phenomena which can lead to the observation of spurious effects or device-dependent issues in the images produced. 2. Using this understanding to optimise the experimental procedures employed in order to reduce these issues and standardise acquisition protocols as a first step towards reproducibility, both within and between institutions/users. 3. Developing freely available (open-access) image calibration and correction protocols for the different image types, based on the use of the standardised acquisition protocols and an understanding of the experimental factors and phenomena, facilitating their intercomparison and interpretation. Experimental factors Phenomena el Figure 1-5. Iterative approach towards a methodology which produces device-independent images and allows better reproducibility and inter-comparisons between images. The approach is summarised in Figure 1-5, which also emphasizes the synergistic and iterative nature of the methodology. The demands of the image correction protocols and the development of the post-processing system will determine the data required and the manner in which it needs to be collected. This in turn impinges on the experimental procedure. In the following sections, the experimental factors and physical phenomena which may lead to spurious effects in the image sets are described, and experimental and post-processing methodologies towards their correction are proposed. The inherent difference in the properties of images capturing reflected radiation versus those that capture photo-induced luminescence also mean that the factors affecting reproducibility and device dependence of images are most easily considered separately. Version No. 1.0 Date : 14/10/2013
